Journal: Cell death discovery

Article Title: Novel meriolin derivatives potently inhibit cell cycle progression and transcription in leukemia and lymphoma cells via inhibition of cyclin-dependent kinases (CDKs).

doi: 10.1038/s41420-024-02056-6

Figure Lengend Snippet: Fig. 1 Meriolin 16 and meriolin 36 are highly cytotoxic to myeloid and lymphoid cell lines and also to primary malignant patient cells. A Cytotoxicity was determined after 24 h with increasing concentrations of meriolin 16 and meriolin 36 in two myeloid cell lines (K562 (chronic myeloid leukemia; CML) and HL60 (acute myeloid leukemia; AML)) and six lymphoid cell lines (SUPB15 (B cell acute lymphoblastic leukemia; B-ALL), KOPTK1 (T-ALL), HPBALL (T cell acute lymphoblastic leukemia; T-ALL), MOLT4 (T-ALL), Ramos (Burkitt B cell lymphoma) and Jurkat (acute T cell lymphoblastic leukemia; T-ALL)). Cell viability was assessed by AlamarBlue® viability assay. Error bars = Mean ± SD of a representative experiment performed in triplicates. B Cytotoxicity was determined after 24 h with increasing concentrations of meriolin 16 and meriolin 36 in malignant primary patient cells such as diffuse large B cell lymphoma cells (DLBCL), follicular lymphoma cells (FL) and chronic lymphocytic leukemia cells (CLL) by AlamarBlue® assay. Error bars = Mean ± SD of a representative experiment performed in triplicates. The respective IC50 values are given on the right side for the respective compound in each cell line. n.d. = not detected in the depicted concentration range.

Article Snippet: HeLa cells (human cervix carcinoma; #ACC-57), HL60 (human acute myeloid leukemia; #ACC-3), HPBALL (human T cell acute lymphoblastic leukemia; #ACC-483), MOLT4 (human T cell acute lymphoblastic leukemia; #ACC-362), K562 (human chronic myeloid leukemia; #ACC-10) and SUPB15 (human B cell acute lymphoblastic leukemia; #ACC-389) were obtained from DSMZ.

Techniques: Viability Assay, Alamar Blue Assay, Concentration Assay

Journal: The Journal of International Medical Research

Article Title: Prognostic impact of miR-196a/b expression in adult acute myeloid leukaemia: a single-centre, retrospective cohort study

doi: 10.1177/0300060518777399

Figure Lengend Snippet: Overexpression of miR-196a/b enhances cellular proliferation, while knockdown of miR-196a/b inhibits HL60 cellular proliferation

Article Snippet: HL60 cell viability was evaluated by the Cell Counting Kit-8 assay (Beyotime) according to the manufacturer’s instructions.

Techniques: Over Expression, Knockdown

Journal:

Article Title: Approach to Validating an Opsonophagocytic Assay for Streptococcus pneumoniae

doi: 10.1128/CDLI.12.2.287-295.2005

Figure Lengend Snippet: Effect of HL60 cell age (expressed in cell passage) on opsonophagocytic activity. Dilutions of a serum specimen from a nonvaccinated adult were tested in the pneumococcal serotype 6B OPA with day 3 HL60 cells (400:1) from postdifferentiated passage 51 (p51; solid line) and 111 (p111; dotted line). Incomplete opsonophagocytic activity (i.e., high baseline numbers of CFU [>20]) was observed when high-passage-number (>80) differentiated HL60 cells were used. However, the OPA titer assigned to the serum specimen remained the same when qualified HL60 cells from a different passage were used.

Article Snippet: The viabilities of the differentiated HL60 cells were assessed by trypan blue exclusion and annexin V-propidium iodide staining (Caltag).

Techniques: Activity Assay

Journal:

Article Title: Approach to Validating an Opsonophagocytic Assay for Streptococcus pneumoniae

doi: 10.1128/CDLI.12.2.287-295.2005

Figure Lengend Snippet: Changes in effector cell/target bacterium ratio on pneumococcal serotype 9V OPA performance. The numbers of CFU obtained in the pneumococcal serotype 9V OPA with one serum specimen are shown by using PMNs or HL60 cells (passage 19) at day 4 postdifferentiation at 400:1 (solid lines) and 50:1 (dotted lines). A single analyst performed the comparison study on the same day using the same bacterial culture to minimize variation.

Article Snippet: The viabilities of the differentiated HL60 cells were assessed by trypan blue exclusion and annexin V-propidium iodide staining (Caltag).

Techniques: Comparison